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  • Featured Product
  • KD/KO Validated

CD63 Polyclonal antibody, PBS Only

CD63 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25682-1-PBS

Synonyms

Ocular melanoma-associated antigen, Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of human urine exosomes tissue using CD63 Polyclonal antibody (25682-1-AP)

    WB analysis of human urine exosomes using 25682-1-AP (same clone as 25682-1-PBS)

    human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human serum exosomes using CD63 Polyclonal antibody (25682-1-AP)

    WB analysis of human serum exosomes using 25682-1-AP (same clone as 25682-1-PBS)

    Human serum exosomes were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD63 Polyclonal antibody (25682-1-AP)

    WB analysis using 25682-1-AP (same clone as 25682-1-PBS)

    Human plasma and human plasma-derived exosomes (human plasma-exo) were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using CD63 Polyclonal antibody (25682-1-AP)

    IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using CD63 Polyclonal antibody (25682-1-AP)

    IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using CD63 Polyclonal antibody (25682-1-AP)

    IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using CD63 Polyclonal antibody (25682-1-AP)

    IHC staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD63 Polyclonal antibody (25682-1-AP)

    IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD63 Polyclonal antibody (25682-1-AP)

    IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD63 Polyclonal antibody (25682-1-AP)

    IHC staining of human tonsillitis using 25682-1-AP (same clone as 25682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD63 Polyclonal antibody (25682-1-AP)

    IHC staining of human lymphoma using 25682-1-AP (same clone as 25682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD63 Polyclonal antibody (25682-1-AP)

    IHC staining of human lymphoma using 25682-1-AP (same clone as 25682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD63 Polyclonal antibody (25682-1-AP)

    IHC staining of human lymphoma using 25682-1-AP (same clone as 25682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25682-1-AP (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human malignant melanoma tissue using CD63 Polyclonal antibody (25682-1-AP)

    IF Staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (25682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human malignant melanoma tissue using CD63 Polyclonal antibody (25682-1-AP)

    IF Staining of human malignant melanoma using 25682-1-AP (same clone as 25682-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (25682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CD63 Polyclonal antibody (25682-1-AP)

    IF Staining of A431 using 25682-1-AP (same clone as 25682-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD63 antibody (25682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25682-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25682-1-PBS targets CD63 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD63 fusion protein Ag19690 相同性解析による交差性が予測される生物種
Full Name CD63 molecule
Calculated molecular weight 26 kDa
Observed molecular weight 28-35 kDa
GenBank accession numberBC002349
Gene symbol CD63
Gene ID (NCBI) 967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.