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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD64 Polyclonal antibody (27563-1-AP)

    WB analysis using 27563-1-AP (same clone as 27563-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using CD64 Polyclonal antibody (27563-1-AP)

    WB analysis of HL-60 using 27563-1-AP (same clone as 27563-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using CD64 Polyclonal antibody (27563-1-AP)

    WB analysis of U-937 using 27563-1-AP (same clone as 27563-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CD64 Polyclonal antibody (27563-1-AP)

    IF Staining of THP-1 using 27563-1-AP (same clone as 27563-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD64 antibody (27563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

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CD64 Polyclonal antibody, PBS Only

CD64 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 27563-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27563-1-PBS targets CD64 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD64 fusion protein Ag26727 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Calculated molecular weight 374 aa, 43 kDa
Observed molecular weight 65-70 kDa, 43-45 kDa
GenBank accession numberBC032634
Gene Symbol FCGR1A
Gene ID (NCBI) 2209
ENSEMBL Gene IDENSG00000150337
RRIDAB_2880909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12314
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.