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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD64 (10.1)

CD64 Monoclonal Antibody for FC, ELISA

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

10.1

Cat no : 65253-1-Ig

Synonyms

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD64 (10.1) (65253-1-Ig)

    FC experiment of human PBMCs using 65253-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD64 (65253-1-Ig, Clone:10.1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD64 (10.1) (65253-1-Ig)

    FC experiment of human PBMCs using 65253-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD64 (65253-1-Ig, Clone:10.1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000, or CoraLite®488-Conjugated Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65253-1-Ig targets CD64 in FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Calculated molecular weight 374 aa, 43 kDa
GenBank accession numberBC032634
Gene symbol FCGR1A
Gene ID (NCBI) 2209
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.

Protocols

Product Specific Protocols
FC protocol for CD64 antibody 65253-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols