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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Atlantic Blue™ Anti-Human CD64 (10.1)

CD64 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Atlantic Blue™ Fluorescent Dye

CloneNo.

10.1

Cat no : AB-65253

Synonyms

CD64, CD64A, Fc gamma RI, Fc gamma RIA, FCG1, FcgammaRIa, FCGR1, FCGR1A, FCRI, FLJ18345, IGFR1, IgG Fc receptor I

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Atlantic Blue™ Anti-Human CD64 (10.1) (AB-65253)

    FC experiment of human PBMCs using AB-65253

    1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD64 (AB-65253, Clone:10.1) or unstained. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Atlantic Blue™ Anti-Human CD64 (10.1) (AB-65253)

    FC experiment of human PBMCs using AB-65253

    1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD64 (AB-65253, Clone:10.1) or unstained. Cells were not fixed. Monocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

AB-65253 targets CD64 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Calculated molecular weight 374 aa, 43 kDa
GenBank accession numberBC032634
Gene symbol FCGR1A
Gene ID (NCBI) 2209
Conjugate Atlantic Blue™ Fluorescent Dye
Excitation/Emission maxima wavelengths404 nm / 458 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.

Protocols

Product Specific Protocols
FC protocol for Atlantic Blue™ CD64 antibody AB-65253Download protocol
Standard Protocols
Click here to view our Standard Protocols