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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD68 Recombinant monoclonal antibody (83014-5-RR)

    WB analysis using 83014-5-RR (same clone as 83014-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83014-5-RR (CD68 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD68 Recombinant monoclonal antibody (83014-5-RR)

    IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD68 Recombinant monoclonal antibody (83014-5-RR)

    IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD68 Recombinant monoclonal antibody (83014-5-RR)

    IHC staining of mouse liver using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD68 Recombinant monoclonal antibody (83014-5-RR)

    IHC staining of mouse liver using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using CD68 Recombinant monoclonal antibody (83014-5-RR)

    IHC staining of rat small intestine using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using CD68 Recombinant monoclonal antibody (83014-5-RR)

    IHC staining of rat small intestine using 83014-5-RR (same clone as 83014-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD68 Recombinant monoclonal antibody (83014-5-RR)

    IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD68 Recombinant monoclonal antibody (83014-5-RR)

    IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat small intestine tissue using CD68 Recombinant monoclonal antibody (83014-5-RR)

    IF Staining of rat small intestine using 83014-5-RR (same clone as 83014-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), CD20 antibody (31909-1-AP, Magenta), EPCAM/CD326 antibody (32189-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83014-5-RR

    Affinity and Kinetic Characterization of 83014-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83014-5-RR against Mouse CD68 were performed. The affinity constant is 8.77 nM.

Mouse CD68 Recombinant monoclonal antibody, PBS Only

CD68 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 83014-5-PBS
Clone No.230504G10

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

230504G10, CD68 antigen, gp110, Scard1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83014-5-PBS targets CD68 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0787

Product name: Recombinant mouse CD68 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 21-291 aa of NM_001291058.1

Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS

相同性解析による交差性が予測される生物種
Full Name CD68 antigen
Calculated molecular weight35 kDa
Observed molecular weight100-120 kDa
GenBank accession numberNM_001291058.1
Gene Symbol Cd68
Gene ID (NCBI) 12514
RRIDAB_3670757
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP31996-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. Due to heavy glycosylation, the apparent molecular mass of CD68 detected by Western blot is generally observed between 80 and 140 kDa, far exceeding its unmodified core protein weight of ~34-37 kDa.

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