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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD68 Recombinant antibody

CD68 Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

230504G10

Cat no : 83014-5-RR

Synonyms

Scard1, gp110, CD68 antigen, 230504G10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD68 Recombinant antibody (83014-5-RR)

    WB analysis using 83014-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83014-5-RR (CD68 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD68 Recombinant antibody (83014-5-RR)

    IHC staining of mouse spleen using 83014-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD68 Recombinant antibody (83014-5-RR)

    IHC staining of mouse spleen using 83014-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD68 Recombinant antibody (83014-5-RR)

    IHC staining of mouse liver using 83014-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CD68 Recombinant antibody (83014-5-RR)

    IHC staining of mouse liver using 83014-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD68 Recombinant antibody (83014-5-RR)

    IF Staining of mouse spleen using 83014-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CD68 Recombinant antibody (83014-5-RR)

    IF Staining of mouse spleen using 83014-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83014-5-RR

    Affinity and Kinetic Characterization of 83014-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83014-5-RR against Mouse CD68 were performed. The affinity constant is 8.77 nM.

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Tested Applications

Positive WB detected inRAW 264.7 cells, J774A.1 cells, NR8383 cells
Positive IHC detected inmouse spleen tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83014-5-RR is CD68 Fusion Protein expressed in E. coli.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD68 antigen
Calculated molecular weight35 kDa
Observed molecular weight100-120 kDa
GenBank accession numberNM_001291058.1
Gene symbol Cd68
Gene ID (NCBI) 12514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
WB protocol for CD68 antibody 83014-5-RRDownload protocol
IHC protocol for CD68 antibody 83014-5-RRDownload protocol
IF protocol for CD68 antibody 83014-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols