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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Mouse CD68 Rabbit Recombinant Antibody

CD68 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230504G5

Cat no : CL488-98029

Synonyms

Scard1, gp110, CD68 antigen, 230504G5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse peritoneal macrophages using CoraLite® Plus 488 Anti-Mouse CD68 Rabbit Recombin (CL488-98029)

    FC experiment of mouse peritoneal macrophages using CL488-98029

    1x10^6 mouse peritoneal macrophages were intracellularly stained with APC Anti-Mouse CD11b and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD68 Rabbit Recombinant Antibody (CL488-98029, Clone:230504G5), or Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of mouse peritoneal macrophages using CoraLite® Plus 488 Anti-Mouse CD68 Rabbit Recombin (CL488-98029)

    FC experiment of mouse peritoneal macrophages using CL488-98029

    1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD68 Rabbit Recombinant Antibody (CL488-98029, Clone:230504G5)(red), or Isotype Control(blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inmouse peritoneal macrophages

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-98029 is CD68 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD68 antigen
Calculated molecular weight35 kDa
GenBank accession numberNM_001291058.1
Gene symbol Cd68
Gene ID (NCBI) 12514
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD68 antibody CL488-98029Download protocol
Standard Protocols
Click here to view our Standard Protocols