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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750-conjugated CD68 Monoclonal antibody

CD68 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

3A9A7

Cat no : CL750-66231

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated CD68 Monoclonal anti (CL750-66231)

    WB analysis using CL750-66231

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66231 (CD68 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inU-937 cells, RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-66231 targets CD68 in WB applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD68 fusion protein Ag22815 相同性解析による交差性が予測される生物種
Full Name CD68 molecule
Calculated molecular weight 37 kDa
Observed molecular weight70-100 kDa
GenBank accession numberBC015557
Gene symbol CD68
Gene ID (NCBI) 968
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 CD68 antibody CL750-66231Download protocol
Standard Protocols
Click here to view our Standard Protocols