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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD71 Recombinant antibody

CD71 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2P14

Cat no : CL488-81793-2

Synonyms

TFR1, TFR, T9, sTfR, p90

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CoraLite® Plus 488-conjugated CD71 Recombinant ant (CL488-81793-2)

    IF Staining of K-562 using CL488-81793-2

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 CD71 antibody (CL488-81793-2, Clone: 2P14 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-81793-2 targets CD71 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD71 fusion protein Ag0123 相同性解析による交差性が予測される生物種
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC001188
Gene symbol CD71
Gene ID (NCBI) 7037
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD71 antibody CL488-81793-2Download protocol
Standard Protocols
Click here to view our Standard Protocols