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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FITC Plus Anti-Human CD71 (OKT9)

CD71 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (2)

Applications

IF, FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

OKT9

Cat no : FITC-65235

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Human PBMC using FITC Plus Anti-Human CD71 (OKT9) (FITC-65235)

    FC experiment of Human PBMC using FITC-65235

    1X10^6 PHA-activated human PBMC were surface stained with 5 ul FITC Plus Anti-Human CD71 (FITC-65235, Clone: OKT9). Unactivated human PBMC were surface stained with 5ul FITC Plus Anti-human CD71 as the negative control. Cells were not fixed.

  • Flow cytometry (FC) experiment of Human PBMC using FITC Plus Anti-Human CD71 (OKT9) (FITC-65235)

    FC experiment of Human PBMC using FITC-65235

    1X10^6 PHA-activated human PBMC were surface stained with 5 ul FITC Plus Anti-Human CD71 (FITC-65235, Clone: OKT9) or Mouse IgG1 Isotype Control. Cells were not fixed.

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Tested Applications

Positive FC detected inHuman PBMC

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

FITC-65235 targets CD71 in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human T-cell acute lymphoblastic leukemia cells 相同性解析による交差性が予測される生物種
Full Name transferrin receptor (p90, CD71)
Calculated molecular weight 85 kDa
GenBank accession numberBC001188
Gene symbol CD71
Gene ID (NCBI) 7037
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD71 antibody FITC-65235Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Cell Chem Biol

Anti-tumor immunotherapy using engineered bacterial outer membrane vesicles fused to lysosome-targeting chimeras mediated by transferrin receptor

Authors - Ling-Yan Su
View Article