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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD72 Monoclonal antibody (CL594-67424)

    IF Staining of human tonsillitis using CL594-67424

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated CD72 antibody (CL594-67424, Clone: 2C1C6 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD72 Monoclonal antibody (CL594-67424)

    IF Staining of human tonsillitis using CL594-67424

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated CD72 antibody (CL594-67424, Clone: 2C1C6 ) at dilution of 1:100.

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CoraLite®594-conjugated CD72 Monoclonal antibody

CD72 Monoclonal Antibody for IF-P
Cat No. CL594-67424
Clone No.2C1C6

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF-P

Synonyms

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67424 targets CD72 in IF-P applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD72 fusion protein Ag14405 相同性解析による交差性が予測される生物種
Full Name CD72 molecule
Calculated molecular weight 359 aa, 40 kDa
GenBank accession numberBC030227
Gene Symbol CD72
Gene ID (NCBI) 971
RRIDAB_2920132
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21854
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

Protocols

Product Specific Protocols
IF protocol for CL594 CD72 antibody CL594-67424Download protocol
Standard Protocols
Click here to view our Standard Protocols