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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PerCP-Cyanine5.5 Anti-Human CD73 (AD2) Mouse IgG2a Recombinant Antibody

CD73 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

AD2

Cat no : CPY5-65564

Synonyms

NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73)

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using PerCP-Cyanine5.5 Anti-Human CD73 (AD2) Mouse IgG2a (CPY5-65564)

    FC experiment of human PBMCs using CPY5-65564

    1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD73 (AD2) Mouse IgG2a Recombinant Antibody (CPY5-65564, Clone: AD2) or unstained. Cells were then stained with 5 ul CoraLite® Plus 647 Anti-Human CD3 (OKT3) (CL647-65133, Clone: OKT3). Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-65564 targets CD73 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen Pre-B leukemia cell line 相同性解析による交差性が予測される生物種
Full Name 5'-nucleotidase, ecto (CD73)
Calculated molecular weight 29 kDa, 63 kDa
GenBank accession numberBC015940
Gene symbol CD73
Gene ID (NCBI) 4907
Conjugate PerCP-Cyanine5.5 Fluorescent Dye
Excitation/Emission maxima wavelengths482 nm / 690 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Protocols

Product Specific Protocols
FC protocol for PerCP-Cyanine5.5 CD73 antibody CPY5-65564Download protocol
Standard Protocols
Click here to view our Standard Protocols