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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD74 Monoclonal antibody (66390-1-Ig)

    WB analysis using 66390-1-Ig (same clone as 66390-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using CD74 Monoclonal antibody (66390-1-Ig)

    WB analysis of Ramos using 66390-1-Ig (same clone as 66390-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using CD74 Monoclonal antibody (66390-1-Ig)

    WB analysis of Daudi using 66390-1-Ig (same clone as 66390-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD74 Monoclonal antibody (66390-1-Ig)

    IF Staining of Raji using 66390-1-Ig (same clone as 66390-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD74 antibody (66390-1-Ig, Clone: 2D8A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

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CD74 Monoclonal antibody, PBS Only

CD74 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66390-1-PBS
Clone No.2D8A1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Ia antigen-associated invariant chain, HLA-DR antigens-associated invariant chain, HLADG, DHLAG, Class-II-associated invariant chain peptide

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66390-1-PBS targets CD74 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD74 fusion protein Ag25503 相同性解析による交差性が予測される生物種
Full Name CD74 molecule, major histocompatibility complex, class II invariant chain
Calculated molecular weight 34 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC018726
Gene Symbol CD74
Gene ID (NCBI) 972
RRIDAB_2881766
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04233
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165;PMID: 36712241).