CD74 Monoclonal antibody

CD74 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D8A1

Cat no : 66390-1-Ig

Synonyms

CD74, DHLAG, HLADG, Ia GAMMA, Ii, p33


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Tested Applications

Positive WB detected inRaji cells, Ramos cells, Daudi cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66390-1-Ig targets CD74 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD74 fusion protein Ag25503 相同性解析による交差性が予測される生物種
Full Name CD74 molecule, major histocompatibility complex, class II invariant chain
Calculated molecular weight 34 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC018726
Gene symbol CD74
Gene ID (NCBI) 972
RRIDAB_2881766
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CD74 antibody 66390-1-IgDownload protocol
IF protocol for CD74 antibody 66390-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Stem Cell Res Ther

DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis

Authors - S Y Wang
WB

Int Immunol

Helicobacter urease suppresses cytotoxic CD8 + T cell responses through activating Myh9-dependent induction of PD-L1.

Authors - Jian Wu
IF

Neural Regen Res

Single-nuclei RNA sequencing uncovers heterogenous transcriptional signatures in Parkinson's disease associated with nuclear receptor-related factor 1 defect

Authors - Piniel Alphayo Kambey
humanIF

Nat Commun

Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment

Authors - Xiaojian Lu
humanWB

Am J Physiol Cell Physiol

WISP1 induces the expression of macrophage migration inhibitory factor (MIF) in human lung fibroblasts through Src kinases and EGFR-activated signaling pathways

Authors - Maria-Elpida Christopoulou
humanWB

bioRxiv

Proteomic analysis reveals the dominant effect of ipomoeassin F on the synthesis of membrane and secretory proteins in triple-negative breast cancer cells

Authors - Brihget Sicairos