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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD74 Monoclonal antibody

CD74 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D8A1

Cat no : CL488-66390

Synonyms

Ia antigen-associated invariant chain, HLA-DR antigens-associated invariant chain, HLADG, DHLAG, Class-II-associated invariant chain peptide

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CoraLite® Plus 488-conjugated CD74 Monoclonal anti (CL488-66390)

    IF Staining of Raji using CL488-66390

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite® Plus 488 CD74 antibody (CL488-66390, Clone: 2D8A1 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488-conjugated CD74 Monoclonal anti (CL488-66390)

    FC experiment of human PBMCs using CL488-66390

    1x10^6 human PBMCs were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated CD74 Monoclonal antibody (CL488-66390, Clone: 2D8A1) or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4). Cells were co-stained with 0.4 ug CoraLite® Plus 647 Anti-Human CD19 (SJ25C1) (CL647-65145, Clone: SJ25C1). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inRaji cells
Positive FC (Intra) detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66390 targets CD74 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD74 fusion protein Ag25503 相同性解析による交差性が予測される生物種
Full Name CD74 molecule, major histocompatibility complex, class II invariant chain
Calculated molecular weight 34 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC018726
Gene symbol CD74
Gene ID (NCBI) 972
RRIDAB_2883326
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD74 antibody CL488-66390Download protocol
FC protocol for CL Plus 488 CD74 antibody CL488-66390Download protocol
Standard Protocols
Click here to view our Standard Protocols