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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594 Anti-Human CD8 (UCHT4)

CD8 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

UCHT4

Cat no : CL594-65204

Synonyms

CD8A, T-cell surface glycoprotein CD8 alpha chain, p32, MAL, Leu2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite®594 Anti-Human CD8 (UCHT4) (CL594-65204)

    FC experiment of human PBMCs using CL594-65204

    1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 (CL750-65151) and 5 ul CoraLite®594 Anti-Human CD8 (CL594-65204, Clone:UCHT4) or CoraLite®594 Mouse IgG2a Isotype Control (CL594-65208). Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite®594 Anti-Human CD8 (UCHT4) (CL594-65204)

    FC experiment of human PBMCs using CL594-65204

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human CD8 (CL594-65204, Clone:UCHT4) or CoraLite®594 Mouse IgG2a Isotype Control (CL594-65208). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65204 targets CD8 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Thymocytes and Sézary T cells 相同性解析による交差性が予測される生物種
Full Name CD8a molecule
Calculated molecular weight 235 aa, 26 kDa
GenBank accession numberBC025715
Gene symbol CD8A
Gene ID (NCBI) 925
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
FC protocol for CL594 CD8 antibody CL594-65204Download protocol
Standard Protocols
Click here to view our Standard Protocols