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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Biotin-conjugated CD81 Monoclonal antibody

CD81 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC

Conjugate

Biotin

CloneNo.

1G2C6

Cat no : Biotin-66866

Synonyms

CD81, CD81 antigen, CD81 molecule, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using Biotin-conjugated CD81 Monoclonal antibody (Biotin-66866)

    IHC staining of human liver using Biotin-66866

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using Biotin-66866 (CD81 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Biotin-conjugated CD81 Monoclonal antibody (Biotin-66866)

    IHC staining of human liver using Biotin-66866

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using Biotin-66866 (CD81 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:100-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66866 targets CD81 in IHC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD81 fusion protein Ag27298 相同性解析による交差性が予測される生物種
Full Name CD81 molecule
Calculated molecular weight 26 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC002978
Gene symbol CD81
Gene ID (NCBI) 975
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Protocols

Product Specific Protocols
IHC protocol for Biotin CD81 antibody Biotin-66866Download protocol
Standard Protocols
Click here to view our Standard Protocols