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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD82 Monoclonal antibody, PBS Only

CD82 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E7A4

Cat no : 66803-1-PBS

Synonyms

CD82, KAI1, Metastasis suppressor Kangai-1, IA4, CD82,KAI1, CD 82

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD82 Monoclonal antibody (66803-1-Ig)

    WB analysis using 66803-1-Ig (same clone as 66803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66803-1-Ig (CD82 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD82 Monoclonal antibody (66803-1-Ig)

    IHC staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD82 Monoclonal antibody (66803-1-Ig)

    IHC staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD82 Monoclonal antibody (66803-1-Ig)

    IHC staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD82 Monoclonal antibody (66803-1-Ig)

    IF Staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD82 antibody (66803-1-Ig, Clone: 1E7A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using CD82 Monoclonal antibody (66803-1-Ig)

    IF Staining of human ovary tumor using 66803-1-Ig (same clone as 66803-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human ovary tumor tissue using CD82 antibody (66803-1-Ig, Clone: 1E7A4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66803-1-PBS targets CD82 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD82 fusion protein Ag17513 相同性解析による交差性が予測される生物種
Full Name CD82 molecule
Calculated molecular weight 30 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC000726
Gene symbol CD82
Gene ID (NCBI) 3732
RRIDAB_2882146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.