CD82 Monoclonal antibody
CD82 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1E7A4
Cat no : 66803-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MCF-7 cells, Jurkat cells, Raji cells, K-562 cells, RAW 264.7 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, human ovary tumor tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
66803-1-Ig targets CD82 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD82 fusion protein Ag17513 相同性解析による交差性が予測される生物種 |
| Full Name | CD82 molecule |
| Calculated molecular weight | 30 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC000726 |
| Gene symbol | CD82 |
| Gene ID (NCBI) | 3732 |
| RRID | AB_2882146 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD82 antibody 66803-1-Ig | Download protocol |
| IHC protocol for CD82 antibody 66803-1-Ig | Download protocol |
| IF protocol for CD82 antibody 66803-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Proteomics Proteomic profiling of plasma exosomes reveals CD82 involvement in the development of esophageal squamous cell carcinoma. | ||
FEBS Open Bio RAB31 in glioma-derived endothelial cells promotes glioma cell invasion via extracellular vesicle-mediated enrichment of MYO1C | ||
Heliyon Overexpression of RBM15 modulated the effect of trophoblast cells by promoting the binding ability between YTHDF2 and the CD82 3'UTR to decrease the expression of CD82 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Guorong (Verified Customer) (11-22-2022) | Clear and specific band
 |
