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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated CD82 Monoclonal antibody

CD82 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E7A4

Cat no : CL594-66803

Synonyms

CD82, KAI1, Metastasis suppressor Kangai-1, IA4, CD82,KAI1, CD 82

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD82 Monoclonal antibody (CL594-66803)

    IF Staining of human tonsillitis using CL594-66803

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD82 antibody (CL594-66803, Clone: 1E7A4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite®594-conjugated CD82 Monoclonal antibody (CL594-66803)

    IF Staining of human tonsillitis using CL594-66803

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD82 antibody (CL594-66803, Clone: 1E7A4 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using CoraLite®594-conjugated CD82 Monoclonal antibody (CL594-66803)

    IF Staining of human ovary tumor using CL594-66803

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human ovary tumor tissue using CoraLite®594 CD82 antibody (CL594-66803, Clone: 1E7A4 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using CoraLite®594-conjugated CD82 Monoclonal antibody (CL594-66803)

    IF Staining of human ovary tumor using CL594-66803

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human ovary tumor tissue using CoraLite®594 CD82 antibody (CL594-66803, Clone: 1E7A4 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue, human ovary tumor tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66803 targets CD82 in IF-P applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD82 fusion protein Ag17513 相同性解析による交差性が予測される生物種
Full Name CD82 molecule
Calculated molecular weight 30 kDa
GenBank accession numberBC000726
Gene symbol CD82
Gene ID (NCBI) 3732
RRIDAB_2920042
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.

Protocols

Product Specific Protocols
IF protocol for CL594 CD82 antibody CL594-66803Download protocol
Standard Protocols
Click here to view our Standard Protocols