• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD83 (HB15e)

CD83 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

FC

Conjugate

Unconjugated

CloneNo.

HB15e

Cat no : 65171-1-Ig

Synonyms

Cell surface protein HB15, CD83 molecule, CD83 antigen, BL11, B-cell activation protein

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of Human Monocytes-derived dendritic cells using Anti-Human CD83 (HB15e) (65171-1-Ig)

    FC experiment of Human Monocytes-derived dendritic cells using 65171-1-Ig

    1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 0.5 ug Anti-Human CD83 (65171-1-Ig, Clone:HB15e) and FITC anti-Mouse IgG1 Antibody at dilution 1:50 (green), or stained with 0.5 ug mouse IgG1 isotype control and FITC anti-Mouse IgG1 Antibody at dilution 1:50 (black). Cells were not fixed.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive FC detected inHuman monocytes-derived dendritic cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65171-1-Ig targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

Tested Reactivity human, non-human primates
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human CD83-transfected Cos cells 相同性解析による交差性が予測される生物種
Full Name CD83 molecule
Calculated molecular weight 205 aa, 23 kDa
GenBank accession numberBC030830
Gene symbol CD83
Gene ID (NCBI) 9308
RRIDAB_2918447
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD83 is a member of the immunoglobulin (Ig) superfamily, consisting of an extracellular V-type Ig-like domain, a transmembrane domain, and a cytoplasmic tail. CD83 is one of the most prominent markers for fully matured dendritic cells (DCs) (PMID: 17334966). It is also found on the surface of other activated hematopoietic cells, including lymphocytes, monocytes, macrophages, and neutrophils (PMID: 31231400; 17334966). CD83 regulates the maturation, activation, and homeostasis of numerous immune cells (PMID: 31231400; 32362900). CD83 can also expressed as a soluble form. Soluble CD83 can bind to DCs and inhibit their maturation (PMID: 17334966; 12403928).

Protocols

Product Specific Protocols
FC protocol for CD83 antibody 65171-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols