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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

APC Anti-Human CD83 (HB15e)

CD83 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1 kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

HB15e

Cat no : APC-65171

Synonyms

B cell activation protein, BL11, CD83, CD83 antigen, CD83 molecule, Cell surface protein HB15, HB15, hCD83

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Human Monocytes-derived dendritic cells using APC Anti-Human CD83 (HB15e) (APC-65171)

    FC experiment of Human Monocytes-derived dendritic cells using APC-65171

    1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 10 ul APC Anti-Human CD83 (APC-65171, Clone:HB15e) (blue) or APC mouse IgG1 isotype control (black). Cells were not fixed.

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Tested Applications

Positive FC detected inHuman monocytes-derived dendritic cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65171 targets CD83 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Reactivity Human, Non-human primates
Host / Isotype Mouse / Mouse IgG1 kappa
Class Monoclonal
Type Antibody
Immunogen Human CD83-transfected Cos cells 相同性解析による交差性が予測される生物種
Full Name CD83 molecule
Calculated molecular weight 205 aa, 23 kDa
GenBank accession numberBC030830
Gene symbol CD83
Gene ID (NCBI) 9308
RRIDAB_2918931
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Protocols

Product Specific Protocols
FC protocol for APC CD83 antibody APC-65171Download protocol
Standard Protocols
Click here to view our Standard Protocols