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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CD85j / LILRB1 (GHI/75)

CD85j / LILRB1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/75

Cat no : 65238-1-Ig

Synonyms

LILRB1, ILT2, ILT 2, FLJ37515, CD85j

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD85j / LILRB1 (GHI/75) (65238-1-Ig)

    FC experiment of human PBMCs using 65238-1-Ig

    1X10^6 PBMC were surface stained with 0.2 ug Anti-Human LILRB1 (65238-1-Ig, Clone: GHI/75) and APC-Donkey anti-Mouse IgG at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Monocytes are gated. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD85j / LILRB1 (GHI/75) (65238-1-Ig)

    FC experiment of human PBMCs using 65238-1-Ig

    1X10^6 PBMC were surface stained with 0.2 ug Anti-Human LILRB1 (65238-1-Ig, Clone: GHI/75) and APC-Donkey anti-Mouse IgG at dilution 1:1000, or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10). Monocytes are gated. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65238-1-Ig targets CD85j / LILRB1 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Human Hairy Cell Leukemia Spleen Cells 相同性解析による交差性が予測される生物種
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Calculated molecular weight 71 kDa
GenBank accession numberBC015731
Gene symbol LILRB1
Gene ID (NCBI) 10859
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.

Protocols

Product Specific Protocols
FC protocol for CD85j / LILRB1 antibody 65238-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols