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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Human CD85j / LILRB1 (GHI/75)

CD85j / LILRB1 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

GHI/75

Cat no : CL647-65238

Synonyms

LILRB1, ILT2, ILT 2, FLJ37515, CD85j

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD85j / LILRB1 (GHI/ (CL647-65238)

    FC experiment of human PBMCs using CL647-65238

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD85j / LILRB1 (CL647-65238, Clone:GHI/75) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD85j / LILRB1 (GHI/ (CL647-65238)

    FC experiment of human PBMCs using CL647-65238

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD85j / LILRB1 (CL647-65238, Clone:GHI/75) or Mouse IgG2b Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65238 targets CD85j / LILRB1 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Human Hairy Cell Leukemia Spleen Cells 相同性解析による交差性が予測される生物種
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Calculated molecular weight 71 kDa
GenBank accession numberBC015731
Gene symbol LILRB1
Gene ID (NCBI) 10859
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LILRB1, also named as CD85j or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 CD85j / LILRB1 antibody CL647-65238Download protocol
Standard Protocols
Click here to view our Standard Protocols