Validation Data Gallery
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Tested Applications
| Positive FC detected in | LPS-stimulated Wistar rat splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
| FC | See 1 publications below |
Product Information
65224-1-Ig targets CD86 in IF, FC applications and shows reactivity with rat samples.
| Tested Reactivity | rat |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | HTLV-1-transformed Lewis-S1 rat T cell line 相同性解析による交差性が予測される生物種 |
| Full Name | CD86 molecule |
| Calculated molecular weight | 36 kDa |
| GenBank accession number | NM_020081 |
| Gene Symbol | Cd86 |
| Gene ID (NCBI) | 56822 |
| RRID | AB_3670278 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | O35531 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD86 antibody 65224-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Prostaglandins Other Lipid Mediat Protective role of Yougui Yin in experimental knee osteoarthritis: from the perspective of macrophage polarization |