CoraLite® Plus 750 Anti-Rat CD86 (24F)

CD86 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rat

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

24F

Cat no : CL750-65224

Synonyms

B7 2, Cd86, CD86 molecule


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Tested Applications

Positive FC detected inLPS-stimulated rat splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65224 targets CD86 in FC applications and shows reactivity with Rat samples.

Tested Reactivity Rat
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen HTLV-1-transformed Lewis-S1 rat T cell line 相同性解析による交差性が予測される生物種
Full Name CD86 molecule
Calculated molecular weight 36 kDa
GenBank accession numberNM_020081
Gene symbol Cd86
Gene ID (NCBI) 56822
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Protocols

Product Specific Protocols
FC protocol for CL Plus 750 CD86 antibody CL750-65224Download protocol
Standard Protocols
Click here to view our Standard Protocols