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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD86 Recombinant antibody, PBS Only

CD86 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240429G12

Cat no : 83523-4-PBS

Synonyms

BU63, B70, B7 2, Activation B7-2 antigen, Activation B7 2 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD86 Recombinant antibody (83523-4-RR)

    WB analysis using 83523-4-RR (same clone as 83523-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83523-4-RR (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD86 Recombinant antibody (83523-4-RR)

    IHC staining of human ovary cancer using 83523-4-RR (same clone as 83523-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83523-4-RR (CD86 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD86 Recombinant antibody (83523-4-RR)

    IHC staining of human ovary cancer using 83523-4-RR (same clone as 83523-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83523-4-RR (CD86 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83523-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83523-4-RR

    Affinity and Kinetic Characterization of 83523-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83523-4-RR against Human CD86 were performed. The affinity constant is 0.766 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83523-4-PBS is CD86 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_175862
Gene symbol CD86
Gene ID (NCBI) 942
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.