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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD86 Recombinant antibody

CD86 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240429G12

Cat no : 83523-4-RR

Synonyms

BU63, B70, B7 2, Activation B7-2 antigen, Activation B7 2 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD86 Recombinant antibody (83523-4-RR)

    WB analysis using 83523-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83523-4-RR (CD86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD86 Recombinant antibody (83523-4-RR)

    IHC staining of human ovary cancer using 83523-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83523-4-RR (CD86 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CD86 Recombinant antibody (83523-4-RR)

    IHC staining of human ovary cancer using 83523-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83523-4-RR (CD86 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83523-4-RR

    Affinity and Kinetic Characterization of 83523-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83523-4-RR against Human CD86 were performed. The affinity constant is 0.766 nM.

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Tested Applications

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83523-4-RR is CD86 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_175862
Gene symbol CD86
Gene ID (NCBI) 942
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Protocols

Product Specific Protocols
WB protocol for CD86 antibody 83523-4-RRDownload protocol
IHC protocol for CD86 antibody 83523-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols