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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PE Anti-Human CD86 Rabbit Recombinant Antibody

CD86 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

240429C6

Cat no : PE-98043

Synonyms

BU63, B70, B7 2, Activation B7-2 antigen, Activation B7 2 antigen

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using PE Anti-Human CD86 Rabbit Recombinant Antibody (PE-98043)

    FC experiment of human PBMCs using PE-98043

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody, and 5 ul PE Anti-Human CD86 Rabbit Recombinant Antibody (PE-98043, Clone: 240429C6) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using PE Anti-Human CD86 Rabbit Recombinant Antibody (PE-98043)

    FC experiment of human PBMCs using PE-98043

    1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD86 Rabbit Recombinant Antibody (PE-98043, Clone: 240429C6) (red) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of PE-98043 is CD86 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
GenBank accession numberNM_175862
Gene symbol CD86
Gene ID (NCBI) 942
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Protocols

Product Specific Protocols
FC protocol for PE CD86 antibody PE-98043Download protocol
Standard Protocols
Click here to view our Standard Protocols