CoraLite® Plus 488 Anti-Human CD8a (OKT8)
CD8a Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Mouse / IgG2a, kappa
Reactivity
Human
Applications
IF/ICC, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
OKT8
Cat no : CL488-65135
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | human peripheral blood mononuclear cells |
| Positive FC detected in | human peripheral blood lymphocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-65135 targets CD8a in IF/ICC, FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Human thymocytes 相同性解析による交差性が予測される生物種 |
| Full Name | CD8a molecule |
| Calculated molecular weight | 235 aa, 26 kDa |
| GenBank accession number | BC025715 |
| Gene symbol | CD8A |
| Gene ID (NCBI) | 925 |
| RRID | AB_2883197 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD8a antibody CL488-65135 | Download protocol |
| FC protocol for CL Plus 488 CD8a antibody CL488-65135 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
