Cardinal Red™ Anti-Human CD8a (RPA-T8)
CD8a Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
human
Applications
FC
Conjugate
Cardinal Red™ Fluorescent Dye
CloneNo.
RPA-T8
Cat no : CR-65144
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CR-65144 targets CD8a in FC applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | N/A 相同性解析による交差性が予測される生物種 |
Full Name | CD8a molecule |
Calculated molecular weight | 235 aa, 26 kDa |
GenBank accession number | BC025715 |
Gene symbol | CD8A |
Gene ID (NCBI) | 925 |
Conjugate | Cardinal Red™ Fluorescent Dye |
Excitation/Emission maxima wavelengths | 592 nm / 611 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. |
Background Information
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
Protocols
Product Specific Protocols | |
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FC protocol for Cardinal Red™ CD8a antibody CR-65144 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |