PE Anti-Mouse CD8a (5H10-1)
CD8a Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2b, lambda
Reactivity
Mouse
Applications
FC
Conjugate
PE Fluorescent Dye
CloneNo.
5H10-1
Cat no : PE-65205
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | C57BL/6 mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65205 targets CD8a in FC applications and shows reactivity with Mouse samples.
| Tested Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Concanavalin A-stimulated BALB/c splenic T cells 相同性解析による交差性が予測される生物種 |
| Full Name | CD8 antigen, alpha chain |
| GenBank accession number | BC030679 |
| Gene symbol | Cd8a |
| Gene ID (NCBI) | 12525 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 496 nm, 565 nm / 578 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD8a antibody PE-65205 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
