CoraLite® Plus 488-conjugated CDC25B Monoclonal antibody
CDC25B Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1H3A4
Cat no : CL488-67145
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | Jurkat cells |
Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-67145 targets CDC25B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | CDC25B fusion protein Ag27193 相同性解析による交差性が予測される生物種 |
Full Name | cell division cycle 25 homolog B (S. pombe) |
Calculated molecular weight | 65 kDa |
Observed molecular weight | 69 kDa |
GenBank accession number | BC006395 |
Gene symbol | CDC25B |
Gene ID (NCBI) | 994 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDC25B, also named as CDC25HU2, belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25B directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 CDC25B antibody CL488-67145 | Download protocol |
FC protocol for CL Plus 488 CDC25B antibody CL488-67145 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |