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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDC27; APC3 Monoclonal antibody, PBS Only

CDC27; APC3 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F10G2

Cat no : 67239-1-PBS

Synonyms

ANAPC3, APC3, CDC27, CDC27 homolog, CDC27; APC3, CDC27Hs, D0S1430E, D17S978E, H NUC, HNUC

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of Jurkat using 67239-1-Ig (same clone as 67239-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of HSC-T6 using 67239-1-Ig (same clone as 67239-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of 4T1 using 67239-1-Ig (same clone as 67239-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of HEK-293 using 67239-1-Ig (same clone as 67239-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IHC staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IF Staining of HEK-293 using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IF Staining of human lung cancer using 67239-1-Ig (same clone as 67239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67239-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67239-1-PBS targets CDC27; APC3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CDC27; APC3 fusion protein Ag25588 相同性解析による交差性が予測される生物種
Full Name cell division cycle 27 homolog (S. cerevisiae)
Calculated molecular weight 92 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC011656
Gene symbol CDC27
Gene ID (NCBI) 996
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.