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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDC27; APC3 Monoclonal antibody

CDC27; APC3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F10G2

Cat no : 67239-1-Ig

Synonyms

CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of Jurkat using 67239-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of HSC-T6 using 67239-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of 4T1 using 67239-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    WB analysis of HEK-293 using 67239-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IHC staining of human lung cancer using 67239-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IHC staining of human lung cancer using 67239-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67239-1-Ig (CDC27; APC3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IF Staining of human lung cancer using 67239-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IF Staining of human lung cancer using 67239-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    IF Staining of HEK-293 using 67239-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CDC27; APC3 antibody (67239-1-Ig, Clone: 2F10G2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of K-562 cells using CDC27; APC3 Monoclonal antibody (67239-1-Ig)

    FC experiment of K-562 using 67239-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human CDC27; APC3 (67239-1-Ig, Clone:2F10G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HSC-T6 cells, 4T1 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67239-1-Ig targets CDC27; APC3 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CDC27; APC3 fusion protein Ag25588 相同性解析による交差性が予測される生物種
Full Name cell division cycle 27 homolog (S. cerevisiae)
Calculated molecular weight 92 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC011656
Gene symbol CDC27
Gene ID (NCBI) 996
RRIDAB_2882518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

Protocols

Product Specific Protocols
WB protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
IHC protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
IF protocol for CDC27; APC3 antibody 67239-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols