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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CDC27; APC3 Monoclonal antibody

CDC27; APC3 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F10G2

Cat no : CL488-67239

Synonyms

CDC27, CDC27 homolog, APC3, APC 3, Anaphase-promoting complex subunit 3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of K-562 cells using CoraLite® Plus 488-conjugated CDC27; APC3 Monoclon (CL488-67239)

    FC experiment of K-562 using CL488-67239

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CDC27; APC3 (CL488-67239, Clone:2F10G2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67239 targets CDC27; APC3 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CDC27; APC3 fusion protein Ag25588 相同性解析による交差性が予測される生物種
Full Name cell division cycle 27 homolog (S. cerevisiae)
Calculated molecular weight 92 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC011656
Gene symbol CDC27
Gene ID (NCBI) 996
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC27/APC3 is a component of the anaphase-promoting complex (APC/cyclosome), which is composed of eight subunits and highly conserved in eukaryotic cells. The APC/cyclosome complex acts as a cell cycle-regulated E3 ubiquitin ligase which mediates ubiquitination and subsequent degradation of target proteins, and lead to the progression control through mitosis and the G1 phase of the cell cycle.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CDC27; APC3 antibody CL488-67239Download protocol
Standard Protocols
Click here to view our Standard Protocols