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  • Featured Product
  • KD/KO Validated

CDC2L1 Polyclonal antibody

CDC2L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28613-1-AP

Synonyms

CDK11, CDK11B, CDK11 p58, CDK11 p46, CDK11 p110

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC2L1 Polyclonal antibody (28613-1-AP)

    WB analysis using 28613-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28613-1-AP (CDC2L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDC2L1 Polyclonal antibody (28613-1-AP)

    WB analysis of HeLa using 28613-1-AP

    WB result of CDC2L1 antibody (28613-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC2L1 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human lung cancer using 28613-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human lung cancer using 28613-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human colon cancer using 28613-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human colon cancer using 28613-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDC2L1 Polyclonal antibody (28613-1-AP)

    IF Staining of HeLa using 28613-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDC2L1 antibody (28613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CDC2L1 Polyclonal antibody (28613-1-AP)

    IF Staining of HUVEC using 28613-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CDC2L1 antibody (28613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, SKOV-3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28613-1-AP targets CDC2L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC2L1 fusion protein Ag28743 相同性解析による交差性が予測される生物種
Full Name cell division cycle 2-like 1 (PITSLRE proteins)
Calculated molecular weight 93 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_001787
Gene symbol CDC2L1
Gene ID (NCBI) 984
RRIDAB_2918181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK11 is encoded by two highly homologous genes known as CDC2L1 and CDC2L2. Due to the distinct structure and alterative RNA splicing, the CDC2L gene can produce three different CDK11 isoforms, a larger 110 kDa protein isoform, a mitosis-specific 58 kDa isoform, and a smaller apoptosis-specific 46 kDa isoform.(PMID 27049727)

Protocols

Product Specific Protocols
WB protocol for CDC2L1 antibody 28613-1-APDownload protocol
IHC protocol for CDC2L1 antibody 28613-1-APDownload protocol
IF protocol for CDC2L1 antibody 28613-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols