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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CDC2L1 Polyclonal antibody

CDC2L1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28613

Synonyms

CDK11, CDK11B, CDK11 p58, CDK11 p46, CDK11 p110

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CoraLite® Plus 488-conjugated CDC2L1 Polyclonal an (CL488-28613)

    IF Staining of HUVEC using CL488-28613

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 CDC2L1 antibody (CL488-28613) at dilution of 1:200, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated CDC2L1 Polyclonal an (CL488-28613)

    FC experiment of HeLa using CL488-28613

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CDC2L1 Polyclonal antibody (CL488-28613)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive IF/ICC detected inHUVEC cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28613 targets CDC2L1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC2L1 fusion protein Ag28743 相同性解析による交差性が予測される生物種
Full Name cell division cycle 2-like 1 (PITSLRE proteins)
Calculated molecular weight 93 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_001787
Gene symbol CDC2L1
Gene ID (NCBI) 984
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK11 is encoded by two highly homologous genes known as CDC2L1 and CDC2L2. Due to the distinct structure and alterative RNA splicing, the CDC2L gene can produce three different CDK11 isoforms, a larger 110 kDa protein isoform, a mitosis-specific 58 kDa isoform, and a smaller apoptosis-specific 46 kDa isoform.(PMID 27049727)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CDC2L1 antibody CL488-28613Download protocol
FC protocol for CL Plus 488 CDC2L1 antibody CL488-28613Download protocol
Standard Protocols
Click here to view our Standard Protocols