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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CDC2L1 Polyclonal antibody (28613-1-AP)

    WB analysis of HeLa using 28613-1-AP (same clone as 28613-1-PBS)

    WB result of CDC2L1 antibody (28613-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC2L1 transfected HeLa cells. This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDC2L1 Polyclonal antibody (28613-1-AP)

    WB analysis using 28613-1-AP (same clone as 28613-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28613-1-AP (CDC2L1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human lung cancer using 28613-1-AP (same clone as 28613-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human lung cancer using 28613-1-AP (same clone as 28613-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human colon cancer using 28613-1-AP (same clone as 28613-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDC2L1 Polyclonal antibody (28613-1-AP)

    IHC staining of human colon cancer using 28613-1-AP (same clone as 28613-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28613-1-AP (CDC2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDC2L1 Polyclonal antibody (28613-1-AP)

    IF Staining of HeLa using 28613-1-AP (same clone as 28613-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDC2L1 antibody (28613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using CDC2L1 Polyclonal antibody (28613-1-AP)

    IF Staining of HUVEC using 28613-1-AP (same clone as 28613-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CDC2L1 antibody (28613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28613-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CDC2L1 Polyclonal antibody, PBS Only

CDC2L1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28613-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28613-1-PBS targets CDC2L1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC2L1 fusion protein Ag28743 相同性解析による交差性が予測される生物種
Full Name cell division cycle 2-like 1 (PITSLRE proteins)
Calculated molecular weight 93 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_001787
Gene Symbol CDC2L1
Gene ID (NCBI) 984
RRIDAB_2918181
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21127
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CDK11 is encoded by two highly homologous genes known as CDC2L1 and CDC2L2. Due to the distinct structure and alterative RNA splicing, the CDC2L gene can produce three different CDK11 isoforms, a larger 110 kDa protein isoform, a mitosis-specific 58 kDa isoform, and a smaller apoptosis-specific 46 kDa isoform.(PMID 27049727)