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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC40 Recombinant antibody (84679-4-RR)

    WB analysis using 84679-4-RR (same clone as 84679-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84679-4-RR (CDC40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84679-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84679-4-RR

    Affinity and Kinetic Characterization of 84679-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84679-4-RR against Human CDC40 were performed. The affinity constant is below 1 pM.

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CDC40 Recombinant antibody, PBS Only

CDC40 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84679-4-PBS
Clone No.242034D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Pre mRNA processing factor 17, hPRP17, EHB3, EH binding protein 3, Cell division cycle 40 homolog

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84679-4-PBS targets CDC40 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDC40 fusion protein Ag36151 相同性解析による交差性が予測される生物種
Full Name cell division cycle 40 homolog (S. cerevisiae)
Observed molecular weight65-67 kDa
GenBank accession numberBC126114
Gene Symbol CDC40
Gene ID (NCBI) 51362
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60508
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CDC40 (cell division cycle 40), also known as EHB3. It is expected to be located in the nucleus, which is ubiquitinated in bone marrow and lymph node. The protein is required for pre-mRNA splicing as component of the activated spliceosome, and it plays an important role in embryonic brain development; this function does not require proline isomerization (PMID: 33220177; 33220177). The molecular weight of CDC40 is 65 kDa.