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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDC40 Recombinant antibody (84679-4-RR)

    WB analysis using 84679-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84679-4-RR (CDC40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84679-4-RR

    Affinity and Kinetic Characterization of 84679-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84679-4-RR against Human CDC40 were performed. The affinity constant is below 1 pM.

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CDC40 Recombinant antibody

CDC40 Recombinant Antibody for WB, ELISA
Cat No. 84679-4-RR
Clone No.242034D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Pre mRNA processing factor 17, hPRP17, EHB3, EH binding protein 3, Cell division cycle 40 homolog

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HUVEC cells, HeLa cells, Jurkat cells, THP-1 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84679-4-RR targets CDC40 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDC40 fusion protein Ag36151 相同性解析による交差性が予測される生物種
Full Name cell division cycle 40 homolog (S. cerevisiae)
Observed molecular weight65-67 kDa
GenBank accession numberBC126114
Gene Symbol CDC40
Gene ID (NCBI) 51362
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60508
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC40 (cell division cycle 40), also known as EHB3. It is expected to be located in the nucleus, which is ubiquitinated in bone marrow and lymph node. The protein is required for pre-mRNA splicing as component of the activated spliceosome, and it plays an important role in embryonic brain development; this function does not require proline isomerization (PMID: 33220177; 33220177). The molecular weight of CDC40 is 65 kDa.

Protocols

Product Specific Protocols
WB protocol for CDC40 antibody 84679-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols