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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

N-cadherin Monoclonal antibody, PBS Only (Detector)

N-cadherin Monoclonal Antibody for WB, IF/ICC, FC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D9G1

Cat no : 68532-2-PBS

Synonyms

CDHN, CDH2, CD325, Cadherin-2, Cadherin 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using N-cadherin Monoclonal antibody (68532-2-Ig)

    WB analysis using 68532-2-Ig (same clone as 68532-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68532-2-Ig (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68532-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using N-cadherin Monoclonal antibody (68532-2-Ig)

    IF Staining of HepG2 using 68532-2-Ig (same clone as 68532-2-PBS)

    Immunofluorescent analysis of un-fixed HepG2 cells using N-cadherin antibody (68532-2-Ig, Clone: 2D9G1 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68532-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using N-cadherin Monoclonal antibody (68532-2-Ig)

    FC experiment of SH-SY5Y using 68532-2-Ig (same clone as 68532-2-PBS)

    1x10^6 MCF-7 (red, dashed) and SH-SY5Y (red, filled) were surface stained with 0.2 μg N-cadherin Monoclonal antibody (68532-2-Ig, Clone: 2D9G1) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were not fixed. This data was developed using the same antibody clone with 68532-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using N-cadherin Monoclonal antibody (68532-2-Ig)

    FC experiment of HepG2 using 68532-2-Ig (same clone as 68532-2-PBS)

    1x10^6 HepG2 cells were surface stained with 0.2 μg N-Cadherin Monoclonal Antibody (68532-2-Ig, Clone: 2D9G1, red) or 0.2 μg Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue), and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1). Cells were not fixed. This data was developed using the same antibody clone with 68532-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50439-1

    Cytometric bead array standard curve of MP50439-1

    Cytometric bead array standard curve of MP50439-1, N-cadherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66219-2-PBS. Detection antibody: 68532-2-PBS. Standard:Eg0701. Range: 0.781-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68532-2-PBS targets N-cadherin as part of a matched antibody pair:

MP50439-1: 66219-2-PBS capture and 68532-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated molecular weight100 kDa
Observed molecular weight130 kDa
GenBank accession numberNM_001792.4
Gene symbol N-cadherin
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).