N-cadherin Monoclonal antibody
N-cadherin Monoclonal Antibody for WB, IF/ICC, FC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IF/ICC, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2D9G1
Cat no : 68532-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | pig heart tissue, rabbit heart tissue, rat heart tissue, mouse heart tissue |
Positive IF/ICC detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68532-2-Ig targets N-cadherin in WB, IF/ICC, FC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
Tested Reactivity | human, mouse, rat, pig, rabbit |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
Full Name | cadherin 2, type 1, N-cadherin (neuronal) |
Calculated molecular weight | 100 kDa |
Observed molecular weight | 130 kDa |
GenBank accession number | NM_001792.4 |
Gene symbol | N-cadherin |
Gene ID (NCBI) | 1000 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).
Protocols
Product Specific Protocols | |
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WB protocol for N-cadherin antibody 68532-2-Ig | Download protocol |
IF protocol for N-cadherin antibody 68532-2-Ig | Download protocol |
FC protocol for N-cadherin antibody 68532-2-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |