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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated CDK1-Specific Polyclonal antibody

CDK1-Specific Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-19532

Synonyms

CDC2, CDK1, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 555-conjugated CDK1-Specific Polycl (CL555-19532)

    FC experiment of MCF-7 using CL555-19532

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human CDK1-Specific (CL555-19532) (red), or 0.2 ug CoraLite® Plus 555 Rabbit IgG Control Antibody (CL555-30000)(Blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-19532 targets CDK1-Specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cell division cycle 2, G1 to S and G2 to M
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberNM_001786
Gene symbol CDK1
Gene ID (NCBI) 983
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CDK1-Specific antibody CL555-19532Download protocol
Standard Protocols
Click here to view our Standard Protocols