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KD/KO Validated

CDK2 Polyclonal antibody

Name: CDK2 Polyclonal antibody
Brand: Proteintech
SKU: 10122-1-AP
Rating: 5.00 (1 reviews)

CDK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

288

Reviews (1)

Cat no : 10122-1-AP

Synonyms

p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2

Validation Data Gallery

Filter:

AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 10122-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 10122-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10122-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10122-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10122-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 10122-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10122-1-AP
IP result of anti-CDK2 (IP:10122-1-AP, 4ug; Detection:10122-1-AP 1:10000) with HeLa cells lysate 1200 ug.
IHC staining of human liver cancer using 10122-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10122-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 10122-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10122-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10122-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10122-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 10122-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 10122-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CDK2 antibody (10122-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10122-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (10122-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
FC experiment of HepG2 using 10122-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CDK2 (10122-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 10122-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, MCF-7 cells, A431 cells, NIH/3T3 cells, rat kidney tissue, HEK-293 cells, Hela cells, Jurkat cells, K-562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse testis tissue, human prostate cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 281 publications below
IHC	See 14 publications below
IF	See 2 publications below
IP	See 1 publications below
FC	See 1 publications below
CoIP	See 4 publications below

Product Information

10122-1-AP targets CDK2 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish, sheep, fish, sea cucumbers
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CDK2 fusion protein Ag0166 相同性解析による交差性が予測される生物種
Full Name	cyclin-dependent kinase 2
Calculated molecular weight	33 kDa
Observed molecular weight	30-33 kDa
GenBank accession number	BC003065
Gene symbol	CDK2
Gene ID (NCBI)	1017
RRID	AB_2078556
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CDK2 antibody 10122-1-AP	Download protocol
IHC protocol for CDK2 antibody 10122-1-AP	Download protocol
IF protocol for CDK2 antibody 10122-1-AP	Download protocol
IP protocol for CDK2 antibody 10122-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP	Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis Authors - Saisai Guo KD Validated View Article
human	WB,IHC	Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer. Authors - Xi Jin KD Validated View Article
human	WB	Sci Adv Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer Authors - Atish Mohanty View Article
human	WB	Nat Commun Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. Authors - Xiaoyu Ma View Article
human	WB	J Clin Invest TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. Authors - Zheng Hu View Article
human	WB	Nat Commun Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets. Authors - Jun Lu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Silvia (Verified Customer) (10-19-2021)

It works well for WB

Applications: Western Blot
Cell Tissue Type: Human fibroblasts

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK2 Polyclonal antibody

CDK2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 10122-1-AP

WB analysis using 10122-1-AP

WB analysis of A431 using 10122-1-AP

WB analysis of MCF-7 using 10122-1-AP

WB analysis of NIH/3T3 using 10122-1-AP

WB analysis of rat kidney using 10122-1-AP

IP experiment of HeLa using 10122-1-AP

IHC staining of human liver cancer using 10122-1-AP

IHC staining of mouse testis using 10122-1-AP

IHC staining of mouse testis using 10122-1-AP

IHC staining of human liver cancer using 10122-1-AP

IHC staining of human liver cancer using 10122-1-AP

IHC staining of human prostate cancer using 10122-1-AP

IHC staining of human prostate cancer using 10122-1-AP

IF Staining of mouse testis using 10122-1-AP

IF Staining of HepG2 using 10122-1-AP

FC experiment of HepG2 using 10122-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Acquired resistance to KRAS G12C small-molecule inhibitors via genetic/nongenetic mechanisms in lung cancer

Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway.

TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.

Whole-exome sequencing of alpha-fetoprotein producing gastric carcinoma reveals genomic profile and therapeutic targets.

Reviews

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