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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated CDK2 Polyclonal antibody

CDK2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-10122

Synonyms

p33 protein kinase, EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 555-conjugated CDK2 Polyclonal anti (CL555-10122)

    FC experiment of A431 using CL555-10122

    1X10^6 A431 cells were intracellularly stained with 0.5 ug CoraLite® Plus 555 Anti-Human CDK2 (CL555-10122) (red), or 0.5 ug CL555 Rabbit Isotype Control(CL555-30000)(blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-10122 targets CDK2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK2 fusion protein Ag0166 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 2
Calculated molecular weight 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC003065
Gene symbol CDK2
Gene ID (NCBI) 1017
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 CDK2 antibody CL555-10122Download protocol
Standard Protocols
Click here to view our Standard Protocols