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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK2 Recombinant antibody (83635-5-RR)

    WB analysis using 83635-5-RR (same clone as 83635-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83635-5-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83635-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK2 Recombinant antibody (83635-5-RR)

    IF Staining of HeLa using 83635-5-RR (same clone as 83635-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2 antibody (83635-5-RR, Clone: 240580A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83635-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDK2 Recombinant antibody (83635-5-RR)

    IF Staining of HepG2 using 83635-5-RR (same clone as 83635-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (83635-5-RR, Clone: 240580A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83635-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CDK2 Recombinant antibody (83635-5-RR)

    FC experiment of HeLa using 83635-5-RR (same clone as 83635-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-5-RR, Clone:240580A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83635-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83635-5-RR

    Affinity and Kinetic Characterization of 83635-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83635-5-RR against Human CDK2 were performed. The affinity constant is 3.84 nM.

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CDK2 Recombinant antibody, PBS Only

CDK2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83635-5-PBS
Clone No.240580A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83635-5-PBS targets CDK2 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK2 fusion protein Ag17133 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 2
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003065
Gene Symbol CDK2
Gene ID (NCBI) 1017
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24941
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily. It is dispensable for myelination but is important for adult oligodendrocyte progenitor cell renewal, and could be one of the underlying mechanisms that drive adult progenitors to differentiate and thus regenerate myelin(PMID:21502361). G2 phase CCNA1/CDK2 controls the timing of entry into mitosis by controlling the subsequent activation of CCNB/CDK1, but also has an unexpected role in coordinating the activation of CCNB/CDK1 at the centrosome and in the nucleus(PMID:18372919).