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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK2 Recombinant antibody (83635-5-RR)

    WB analysis using 83635-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83635-5-RR (CDK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK2 Recombinant antibody (83635-5-RR)

    IF Staining of HeLa using 83635-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2 antibody (83635-5-RR, Clone: 240580A6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDK2 Recombinant antibody (83635-5-RR)

    IF Staining of HepG2 using 83635-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK2 antibody (83635-5-RR, Clone: 240580A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CDK2 Recombinant antibody (83635-5-RR)

    FC experiment of HeLa using 83635-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK2 Recombinant antibody (83635-5-RR, Clone:240580A6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83635-5-RR

    Affinity and Kinetic Characterization of 83635-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83635-5-RR against Human CDK2 were performed. The affinity constant is 3.84 nM.

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CDK2 Recombinant antibody

CDK2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83635-5-RR
Clone No.240580A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

EC:2.7.11.22, Cyclin-dependent kinase 2, Cyclin dependent kinase 2, Cell division protein kinase 2, CDKN2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  100 μL, 1000 μg/ml
20 μL, 1000 μg/ml
100 μL, 1000 μg/ml

¥85,000/ 100 μL

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Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells, K-562 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83635-5-RR targets CDK2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CDK2 fusion protein Ag17133 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 2
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003065
Gene Symbol CDK2
Gene ID (NCBI) 1017
RRIDAB_3671247
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24941
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily. It is dispensable for myelination but is important for adult oligodendrocyte progenitor cell renewal, and could be one of the underlying mechanisms that drive adult progenitors to differentiate and thus regenerate myelin(PMID:21502361). G2 phase CCNA1/CDK2 controls the timing of entry into mitosis by controlling the subsequent activation of CCNB/CDK1, but also has an unexpected role in coordinating the activation of CCNB/CDK1 at the centrosome and in the nucleus(PMID:18372919).

Protocols

Product Specific Protocols
WB protocol for CDK2 antibody 83635-5-RRDownload protocol
IF protocol for CDK2 antibody 83635-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols