CDK5 Monoclonal antibody
CDK5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1B9E1
Cat no : 68514-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, PC-3 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68514-1-Ig targets CDK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.
Tested Reactivity | Human, Mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CDK5 fusion protein Ag28465 相同性解析による交差性が予測される生物種 |
Full Name | cyclin-dependent kinase 5 |
Calculated molecular weight | 31 kDa, 33 kDa |
Observed molecular weight | 33 kDa |
GenBank accession number | BC005115 |
Gene symbol | CDK5 |
Gene ID (NCBI) | 1020 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.
Protocols
Product Specific Protocols | |
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WB protocol for CDK5 antibody 68514-1-Ig | Download protocol |
IHC protocol for CDK5 antibody 68514-1-Ig | Download protocol |
IF protocol for CDK5 antibody 68514-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |