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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK5 Monoclonal antibody, PBS Only

CDK5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B9E1

Cat no : 68514-1-PBS

Synonyms

CDK5, Cell division protein kinase 5, cyclin dependent kinase 5, PSSALRE, TPKII catalytic subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK5 Monoclonal antibody (68514-1-Ig)

    WB analysis using 68514-1-Ig (same clone as 68514-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68514-1-Ig (CDK5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68514-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDK5 Monoclonal antibody (68514-1-Ig)

    IHC staining of mouse brain using 68514-1-Ig (same clone as 68514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68514-1-Ig (CDK5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68514-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CDK5 Monoclonal antibody (68514-1-Ig)

    IHC staining of mouse brain using 68514-1-Ig (same clone as 68514-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68514-1-Ig (CDK5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68514-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CDK5 Monoclonal antibody (68514-1-Ig)

    IF Staining of SH-SY5Y using 68514-1-Ig (same clone as 68514-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CDK5 antibody (68514-1-Ig, Clone: 1B9E1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68514-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68514-1-PBS targets CDK5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDK5 fusion protein Ag28465 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 5
Calculated molecular weight 31 kDa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC005115
Gene symbol CDK5
Gene ID (NCBI) 1020
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Cyclin-dependent kinase 5 (CDK5), belongs to the cyclin-dependent kinase family, is a proline-directed serine/threonine-protein kinase that essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. CDK5 predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau.